pstat3  (Bioss)

Journal: iScience

Article Title: Targeting CCR5 with miltefosine as a therapeutic strategy for thrombocytopenia

doi: 10.1016/j.isci.2025.112379

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Article Snippet: Phospho-STAT3 (Ser727) Polyclonal antibody , Proteintech , Cat# 28945-1-AP.

Techniques: Recombinant, Lysis, CCK-8 Assay, Staining, Membrane, Western Blot, Software

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Gastric cancer-derived exosomal let-7 g-5p mediated by SERPINE1 promotes macrophage M2 polarization and gastric cancer progression

doi: 10.1186/s13046-024-03269-4

Figure Lengend Snippet: SERPINE1 -mediated GC-derived exosomal let-7 g-5p facilitates macrophage M2 polarization through STAT3 hyperphosphorylation resulting from inhibition of SOCS7 interactions with STAT3. ( A ) Differential miRNA analysis of exosomes derived from MKN45 cells with stably silenced SERPINE1 and normal MKN45 cells using sRNA-Seq. N, normal group. sh, stably silenced SERPINE1 . ( B ) Venn diagram of target genes predicted by miRDB, miRWalk, and miRTarBase for let-7 g-5p. ( C ) Network of target genes that interact with STAT3. ( D ) KEGG pathway analysis of the 78 target genes of let-7 g-5p using DAVID. ( E ) Schematic representation: exosomal let-7 g-5p ingested by macrophages inhibits SOCS7 interaction with STAT3, resulting in STAT3 hyperphosphorylation. ( F ) Flow cytometric assay of the impact of let-7 g-5p on M2 polarization induced by exosomes derived from GC cells. ( G ) Western blotting analysis for the levels of SOCS7 protein and STAT3 phosphorylation in macrophages treated with exosomes and antagomir-let-7 g-5p. ( H and I ) Endogenous CoIP assay for SOCS7 and STAT3 in macrophages ingesting exosomes derived from normal MKN45 cells. ( J ) Western blotting analysis of SOCS7 protein levels in xenograft tumors

Article Snippet: Antigen retrieval was performed using sodium citrate buffer (pH 6.0, 98 °C), followed by goat serum blocking for 1 h. Sections were incubated with PAI-1 (rabbit, 1:200, Immunoway), CD163 (mouse, 1:200, Immunoway), CD206 (mouse, 1:200, Proteintech), F4/80 (rabbit, 1:200, Bioss), iNOS (rabbit, 1:200, Bioss), Arg1 (rabbit, 1:200, Proteintech), STAT3 (rabbit, 1:200, Bioss) antibodies overnight at 4 °C, reactivated, stained with Cy3-conjugated goat anti-rabbit IgG (Abcam) and Alexa Fluor 488-conjugated goat anti-mouse IgG (Abcam) for 30 min, counterstained with DAPI, and imaged using fluorescence microscopy (IX51, Olympus) for ImageJ analysis.

Techniques: Derivative Assay, Inhibition, Stable Transfection, Flow Cytometry, Western Blot, Co-Immunoprecipitation Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Gastric cancer-derived exosomal let-7 g-5p mediated by SERPINE1 promotes macrophage M2 polarization and gastric cancer progression

doi: 10.1186/s13046-024-03269-4

Figure Lengend Snippet: SERPINE1 promotes exosomal let-7 g-5p expression through the JAK2/STAT3 pathway. ( A ) GSEA was conducted for SERPINE1 co-expressed genes using GSEA (version 4.1.0). ( B ) Heatmap of 35 phosphorylation sites of 34 STAT3 upstream proteins determined by the median fluorescent intensity of the protein array normalized using Grubb’s algorithm. ( C ) Venn diagram of 34 downregulated phosphorylated proteins in the silenced SERPINE1 group and 107 TFs targeting let-7 g-5p. ( D ) Bubble plot combined with Sankey diagram of enriched KEGG pathways for 32 of the 34 STAT3 upstream proteins. ( E ) Statistical analysis of normalized phospho- and nonphospho-fluorescent protein spots in protein arrays. Western blotting analysis of total protein and phosphorylation levels of JAK2/STAT3 in GC cells silencing SERPINE1 ( F ), overexpressing SERPINE1 or treated with a JAK inhibitor ( G ). ( H ) Western blotting analysis of JAK2/STAT3 and SOCS7 in xenograft tumors. ( I ) Representative FISH images and comparison of let-7 g-5p expression in GC cells. ( J ) qRT-PCR analysis of exosomal let-7 g-5p expression in GC cells. ( K ) Representative FISH images and comparison of let-7 g-5p expression in xenograft tissues. ( L ) STAT3-binding motif and sites in the let-7 g-5p promoter region predicted using the JASPR database. ( M ) ChIP-qPCR assay demonstrated that STAT3 interacted with the let-7 g-5p promoter (site position: -1666~-1483). ( N ) Dual-luciferase reporter gene assay for the let-7 g-5p promoter region (position: -1692~-1420). ( O ) Luciferase activity of wt- and mut- let-7 g-5p promoter in the presence of vector or oeSTAT3. Mut, mutated-type. WT, wild-type. Vector, negative control plasmid. oeSTAT3, STAT3 overexpression plasmid

Article Snippet: Antigen retrieval was performed using sodium citrate buffer (pH 6.0, 98 °C), followed by goat serum blocking for 1 h. Sections were incubated with PAI-1 (rabbit, 1:200, Immunoway), CD163 (mouse, 1:200, Immunoway), CD206 (mouse, 1:200, Proteintech), F4/80 (rabbit, 1:200, Bioss), iNOS (rabbit, 1:200, Bioss), Arg1 (rabbit, 1:200, Proteintech), STAT3 (rabbit, 1:200, Bioss) antibodies overnight at 4 °C, reactivated, stained with Cy3-conjugated goat anti-rabbit IgG (Abcam) and Alexa Fluor 488-conjugated goat anti-mouse IgG (Abcam) for 30 min, counterstained with DAPI, and imaged using fluorescence microscopy (IX51, Olympus) for ImageJ analysis.

Techniques: Expressing, Protein Array, Western Blot, Comparison, Quantitative RT-PCR, Binding Assay, Luciferase, Reporter Gene Assay, Activity Assay, Plasmid Preparation, Negative Control, Over Expression

Journal: Cells

Article Title: A Novel Liquid Biopsy Method Based on Specific Combinations of Vesicular Markers Allows Us to Discriminate Prostate Cancer from Hyperplasia

doi: 10.3390/cells13151286

Figure Lengend Snippet: The cluster counts in the tumor and hyperplasia samples for the surface and intra-vesicular targets. The subfigures represent clusters expressing ERBB3 and ALK ( A ); ERBB3, ALK, and CD81 ( B ); STAT3 ( C ); STAT3 and CD81 ( D ); STAT3 and CyclinD1 ( E ); and STAT3, CyclinD1, and CD81 ( F ). Abbreviations: PCa: prostate cancer; BPH: benign prostate hyperplasia. Symbols represent: “*” p < 0.05; “ns” p ≥ 0.05; “•”refer to values out of ±1.5 * IQR.

Article Snippet: Additionally, custom antibodies, such as anti-ErbB-3/HER3-AF ® 555 (Bioss Antibodies, bs-1454R-A555), anti-ALK-AF ® 488 (Bioss Antibodies, bs-0097R-A488), anti-STAT3-AF ® 555 (Bioss Antibodies, bs-3429R-A555), and anti-Cyclin D1-AF ® 488 (Bioss Antibodies, bs-0623R-A488), were used.

Techniques: Expressing

Journal: Cells

Article Title: A Novel Liquid Biopsy Method Based on Specific Combinations of Vesicular Markers Allows Us to Discriminate Prostate Cancer from Hyperplasia

doi: 10.3390/cells13151286

Figure Lengend Snippet: The receiver operating characteristic curves for the intra-vesicular and surface targets. ROC curves for intra-vesicular and surface targets with the most differentiated counts: ERBB3, ALK ( A ); ERBB3, ALK, and CD81 ( B ); STAT3 ( C ); STAT3 and CD81 ( D ); STAT3 and CyclinD1 ( E ); and STAT3, CyclinD1, and CD81 ( F ).

Article Snippet: Additionally, custom antibodies, such as anti-ErbB-3/HER3-AF ® 555 (Bioss Antibodies, bs-1454R-A555), anti-ALK-AF ® 488 (Bioss Antibodies, bs-0097R-A488), anti-STAT3-AF ® 555 (Bioss Antibodies, bs-3429R-A555), and anti-Cyclin D1-AF ® 488 (Bioss Antibodies, bs-0623R-A488), were used.

Techniques:

Journal: Cells

Article Title: A Novel Liquid Biopsy Method Based on Specific Combinations of Vesicular Markers Allows Us to Discriminate Prostate Cancer from Hyperplasia

doi: 10.3390/cells13151286

Figure Lengend Snippet: Contingency tables with cutoff values and performance metrics for markers: ERBB3 and ALK; ERBB3, ALK, and CD81; STAT3; STAT3 and CD81; STAT3 and CyclinD1; and STAT3, CyclinD1, and CD81.

Article Snippet: Additionally, custom antibodies, such as anti-ErbB-3/HER3-AF ® 555 (Bioss Antibodies, bs-1454R-A555), anti-ALK-AF ® 488 (Bioss Antibodies, bs-0097R-A488), anti-STAT3-AF ® 555 (Bioss Antibodies, bs-3429R-A555), and anti-Cyclin D1-AF ® 488 (Bioss Antibodies, bs-0623R-A488), were used.

Techniques:

Journal: Cells

Article Title: A Novel Liquid Biopsy Method Based on Specific Combinations of Vesicular Markers Allows Us to Discriminate Prostate Cancer from Hyperplasia

doi: 10.3390/cells13151286

Figure Lengend Snippet: K-means cluster analysis. The above graph was generated from the analysis of the four most characterizing targets: STAT3; STAT3 and CD81; STAT3 and CyclinD1; and STAT3, CyclinD1, and CD81.

Article Snippet: Additionally, custom antibodies, such as anti-ErbB-3/HER3-AF ® 555 (Bioss Antibodies, bs-1454R-A555), anti-ALK-AF ® 488 (Bioss Antibodies, bs-0097R-A488), anti-STAT3-AF ® 555 (Bioss Antibodies, bs-3429R-A555), and anti-Cyclin D1-AF ® 488 (Bioss Antibodies, bs-0623R-A488), were used.

Techniques: Generated

Journal: Cell Reports Medicine

Article Title: Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors

doi: 10.1016/j.xcrm.2023.101374

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Article Snippet: Rabbit polyclonal anti-phospho-STAT3(Ser727) , CST , Cat#9134T; RRID: AB_331589.

Techniques: Recombinant, Derivative Assay, Microarray, Enzyme-linked Immunosorbent Assay, Lysis, Blocking Assay, Staining, Transfection, Marker, Flow Cytometry, shRNA, Plasmid Preparation, Negative Control, Silver Staining, Bicinchoninic Acid Protein Assay, Selection, Extraction, Labeling, RNA Expression, Cell Culture, Software

Journal: iScience

Article Title: Cancer selective cell death induction by a bivalent CK2 inhibitor targeting the ATP site and the allosteric αD pocket

doi: 10.1016/j.isci.2024.108903

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Article Snippet: STAT3-phospho-Ser727, Rabbit polyclonal antibody , Cell Signaling Technology , Cell Signaling Technology Cat# 9134, RRID: AB_331589.

Techniques: Recombinant, Software